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ORIGINAL ARTICLE
Year : 2019  |  Volume : 8  |  Issue : 9  |  Page : 2937-2941

Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study


1 Department of Rheumatology, King George Medical University, Lucknow, Uttar Pradesh, India
2 Department of Rheumatology, All India Institute of Medical Sciences, New Delhi, India
3 Department of Rheumatology, Post Doctoral Fellow Rheumatology, Christian Medical College, Vellore, Tamil Nadu; Department of Rheumatology, Senior Research Fellow, AIIMS; Consultant Rheumatologist, Sant Parmanand Hospital, Delhi, India

Correspondence Address:
Dr. Aarti Sharma
Department of Rheumatology, All India Institute of Medical Sciences, New Delhi
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jfmpc.jfmpc_471_19

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Method: A cohort study was carried out for a year to evaluate the presence of G-to-A transition in 5′-untranslated region of ankylosis human (ANKH) gene in Indian Khatri patients (closely resembling Europeans of primary knee osteoarthritis (OA), residing in Lucknow, India. Results: In the total participants, 25 were Khatri primary knee OA patients (cases) residing in Lucknow and 101 were random blood donors' samples (controls) collected from a blood bank. All were studied for the abovementioned mutation using real-time polymerase chain reaction (RT-PCR). GG genotype was present in 72.3% of controls and 76% of Khatri knee OA patients. The studied G-to-A mutation was found to be positive in 24.8% of controls and 16% of cases, odds ratio (95% confidence interval) being 0.6 (0.19–1.98, P = 0.42). The frequency of AA (D) genotype found around 3% (cases) and 8% (controls) with P value of 0.70. The combined frequency of both homozygous and heterozygous mutation (GA and AA) in the studied population was 28 (27.7%) in controls and 6 (24%) in cases with the odds ratio (OD) ratio of 0.82 (0.29–2.27, P = 0.70). No significant differences were observed at both genotype and allelic level in the distribution of ANKH-4 G-to-A gene polymorphism in studied subjects. Conclusion: This study did not show any significant G to A mutation in the studied subjects.


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