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ORIGINAL ARTICLE
Year : 2020  |  Volume : 9  |  Issue : 1  |  Page : 125-130

AgNOR as an effective diagnostic tool for determining the proliferative nature of different types of odontogenic cysts


1 Department of Oral and Maxillofacial Pathology, Kathmandu Medical College, Nepal
2 Department of Oral and Maxillofacial Surgery, ESIC Dental College and Hospital, Rohini, Delhi, India
3 Department of Orthodontics & Dentofacial Orthopaedics, Kothiwal Dental College & Research Centre, Moradabad, U.P, India
4 Department of Pedodontics and Preventive Dentistry, Santosh Dental College and Hospital, Ghaziabad, Uttar Pradesh, India
5 Department of Dentistry, Subdivisional Hospital, Bundu, Ranchi, Jharkhand, India
6 Department of Public Health Dentistry, I.T.S Centre for Dental Studies and Research, Muradnagar, Ghaziabad, Uttar Pradesh, India
7 Department of Public Health Dentistry, Post Graduate Institute of Dental Sciences, Rohtak, Haryana, India

Correspondence Address:
Dr. Deepak Passi
Subdivisional Hospital, Bundu, Ranchi, Jharkhand
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jfmpc.jfmpc_617_19

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Objective: To determine the proliferative nature of different types of odontogenic cysts using silver-stained nucleolar organizer region (AgNOR) staining. Materials and Methods: Forty histopathological sections of each of odontogenic keratocyst, dentigerous, and radicular growth were taken into study. Two sections were produced using the paraffin block of separate cases, one was stained with hematoxylin and eosin and another segment was stained with silver nitrate method. Results: AgNOR staining shows black dots and other parts of nuclei show yellow-brown dots. Conclusion: AgNOR, which stain for NOR-related proteins, corresponds to cell multiplication. Their amount regarding the number of AgNORs per cyst can be a good marker of the proliferative action of the cell in aggressive cystic lesion having malignant potential.


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