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ORIGINAL ARTICLE
Year : 2020  |  Volume : 9  |  Issue : 8  |  Page : 3965-3969  

Association of specific microorganisms with endodontic signs and symptoms. A comparative study


1 Department of Psychiatry, Nalanda Medical College, Patna, Bihar, India
2 Department of Conservative Dentistry and Endodontics, Awadh Dental College and Hospital, Jamshedpur, Jharkhand, India
3 Department of Oral Medicine and Radiology, Awadh Dental College and Hospital, Jamshedpur, Jharkhand, India
4 Department of Oral Pathology, Microbiology and Forensic Odontology, Rajendra Institute of Medical Sciences, Dental Institute, Ranchi, Jharkhand, India
5 Department of Conservative Dentistry and Endodontics, Hi-tech Dental College & Hospital, Bhubaneswar, Odisha, India
6 Department of Conservative Dentistry and Endodontics, Rama Dental College and Hospital, Kanpur, UP, India
7 BRS Dental College and Hospital, Sultanpur, Panchkula, Haryana, India

Date of Submission02-Apr-2020
Date of Decision26-Apr-2020
Date of Acceptance02-Jun-2020
Date of Web Publication25-Aug-2020

Correspondence Address:
Dr. Dinesh Kumar
BRS Dental College and Hospital, Sultanpur, Panchkula - 134109, Haryana
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jfmpc.jfmpc_523_20

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  Abstract 


Aim: The study aimed to evaluate the association of root canal microorganisms red complex and E. facealis with endodontic clinical signs and symptoms using polymerase chain reaction. Materials and Methods: Bacterial samples were obtained using sterile paper points from the teeth of 100 subjects divided into two groups; Group I: 50 individuals with primary tooth infections and Group II: 50 individuals with failed endodontic treatment having the secondary infection. DNA extracted from samples was analyzed for endodontic pathogens by using species-specific primers. Results: The pain was noticed in 66%, (33 of 50 subjects) in primary infection and 60% (30 of 50) in a secondary infection. A statistically significant association between pain and E. faecalis bacteria observed both in primary infection and secondary infection (P < 0.05). Tenderness on percussion was associated with 40% cases in Group I and 70% cases in Group II. The red complex accounted for 94% of cases associated with tenderness on percussion in primary infection while 86% of cases associated with secondary infection with a statistically significant association (P < 0.05). Conclusion: Prevalence of red complex bacteria and E. faecalis suggested the association of studied bacteria with symptomatic infected pulp and periradicular diseases.

Keywords: Bacteria, pulp, red complex


How to cite this article:
Singh KK, Kumar P, Das P, Marandi M, Panda S, Mahajan A, Kumar D. Association of specific microorganisms with endodontic signs and symptoms. A comparative study. J Family Med Prim Care 2020;9:3965-9

How to cite this URL:
Singh KK, Kumar P, Das P, Marandi M, Panda S, Mahajan A, Kumar D. Association of specific microorganisms with endodontic signs and symptoms. A comparative study. J Family Med Prim Care [serial online] 2020 [cited 2020 Sep 28];9:3965-9. Available from: http://www.jfmpc.com/text.asp?2020/9/8/3965/293041




  Introduction Top


The presence of bacteria in the root canal leads to the development of periapical periodontitis. Several studies have shown an association between painful exacerbation of periapical lesions and the presence in the root canal of specific bacteria.[1] Based on root canal infection and severity there may be the presence of different microorganisms like Gram-negative anaerobes such as- Prevotella, Veilonella, Porphyromonas; Gram-positive anaerobes such as- Peptostreptococcus, Actinomyces; Gram-negative facultative such as- Eikenella, Hemophilus, and Gram-positive facultative such as- Lactobacillus, Actinobacillus, Propionibacterium, etc.[2]

An infected root canal comprises of a unique microenvironment housing selective microflora. These organisms grow in planktonic forms or as aggregates as well as in biofilms. The microbial composition in the root canal system is an interesting area of research nowadays. Due to advancements in recent years, novel technologies such as- immunological assays and molecular methods like- polymerase chain reaction.[3]

While many different bacterial species can colonize the dental root canals, it has been shown that there is a correlation between the presence of specific bacteria and some endodontic symptoms and signs. Knowledge regarding the nature of endodontic microbiota depends upon the recognition of those microorganisms present in the root canal system of teeth with necrotic pulp and failed endodontic treatment.[4],[5],[6]

The present study aimed to evaluate the correlation between clinical signs and the presence of root canal microorganisms (red complex bacteria and E. faecalis)in an infected root canal.


  Materials and Methods Top


One hundred symptomatic individuals of age group 18 to 65 years undergoing root canal treatment were selected and divided into two groups: Group A: 50 individuals with primary infections, Group B: 50 individuals requiring retreatment with a failed root canal. Inclusion criteria for case selection included- necrotic pulp or periapical periodontitis and clear or purulent discharge. Written informed consent was obtained from all the individuals before the sampling procedure. The study design was approved by the institutional ethical committee (Reg No. IEC/62/BRS/2019).

The following clinical features were recorded for each patient so that they could be correlated with the microbial findings: Pain, tenderness to percussion, swelling, caries, pus discharge, and mobility. In primary infection, the clinical condition of pulp was evaluated by thermal stimuli and vitality tests.

Samples collection was made by inserting a sterile paper point until the complete working length for 1 min. The paper points were then immediately placed into the sterile transport medium and were stored at − 70°C for polymerase chain reaction (PCR). The root canal samples were shaken in a vortex mixer for 60 s. After vortexing, 50 μL of the sample was plated onto selective culture media. Then the paper points were removed. Samples were centrifuged at 2000 g for 5 min. Pellets obtained were suspended in 100 μL of HCl buffer (pH 8.5). Microbial DNA extraction and purification of the samples was performed using GenElute Bacterial Genomic DNA Kit (Sigma–Aldrich, St. Louis, MO, USA) according to the manufacturer's instructions. The DNA content was analyzed using a spectrophotometer.

The PCR program cycle was as under- 94°C for 4 min; 30 cycles at 94°C for 30 s, 55°C for 30 s, 72°C for 30 s, and finally, 72°C for 10 min. Visualization of bands was done under UV illumination using 1% agarose gel electrophoresis employing ethidium bromide.

The data collected from each sample was typed in Microsoft Excel Worksheet 2007 and was analyzed using SPSS 12.0 (SPSS Inc., Chicago, IL).


  Results Top


In the Group, I the clinical characteristics studied were as pain 33 (66%) and tenderness on percussion 40 (80%), but in Group II teeth associated with pain were 30 (60%) and teeth associated with tenderness on percussion were 35 (70%) [Table 1] and [Table 2].
Table 1: Table demonstrating association of red complex and E. faecalis with pain in primary and secondary infections

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Table 2: Table demonstrating association of red complex and E. faecalis with tenderness on percussion in primary and secondary infections

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The pain was noticed in 66%, (33 of 50 subjects) in primary infection and 60% (30 of 50) in a secondary infection. A statistically significant association between pain and E. faecalis bacteria was observed both in primary infection and secondary infection (P < 0.05) by Fisher's exact test [Table 1]. Tenderness on percussion was associated with 40% cases in Group I and 70% cases in Group II. The red complex accounted for 94% of cases associated with tenderness on percussion in primary infection while 86% of cases associated with secondary infection with the statistically significant association (P < 0.05) as evaluated by Fisher's exact test [Table 2].


  Discussion Top


The dental root canal is close to some of the most heavily bacterially contaminated sites in the body. It is extremely likely that a diverse range of species reach the root canal and surrounding dentin. Some early studies failed to report the presence of obligate anaerobes. This can be attributed to the methods employed. Even now, it is salutary to consider that molecular biology has revealed oral species that are currently “uncultivable”. There remains the possibility that studies relying on microbial culture may have overlooked such uncultivable species. Very few investigations have examined the flora of microenvironments of the endodontium. Initially, the presence of microorganisms in the endodontium goes unrecognized. This is because problems associated with a change in the canal's environment only appear when pulpal inflammation is widespread and has reached an advanced state whose outcome is complete pulpal necrosis.[7],[8]

A wide range of species has been reported in endodontic samples. Nevertheless, it is obvious that certain species are more frequently isolated than others and that most infected root canals have a mixed flora.

E. faecalis is found to be the most prevalent microorganism in root canals with persistent periapical lesions. This microorganism can even survive in an environment with scant available nutrients and in which commensality with other bacteria is minimal. Studies by Pirani et al.[9] have demonstrated that E. faecalis can penetrate dentinal tubules, sometimes to a deep extent, which can enable this species to escape the effects of intracanal antimicrobial procedures. Besides, E. faecalis can form biofilms in root canals, and this ability can be important for bacterial resistance and persistence after endodontic procedures. E. faecalis is also resistant to calcium hydroxide, a commonly used intracanal medicament, and such ability to resist high pH values seems to be related to a functioning proton pump, which drives protons into the cell to acidify the cytoplasm.[10],[11],[12]

Although E. faecalis is found in most cases of infected canals, its role, if any, in the pathogenesis of the periapical diseases associated with necrotic pulp or endodontic failure, remains to be elucidated. In our study, there was a significant association between pain and the presence of E. faecalis in both primary and secondary infections.[4]

Rocas et al.[13] have also suggested that E. faecalis was more frequently detected in asymptomatic cases than in symptomatic ones using nested polymerase chain reaction analysis in which they detected E. faecalis in 11.5% of the asymptomatic teeth and 3.7% of symptomatic cases.

Selcuk M. Ozbek et al.[14] investigated the presence of “red complex” in acute periradicular abscesses by using real-time PCR method. At least 1 member of the red complex was found in 84% of the cases. In general, T. denticola, P. gingivalis, and T. forsythia were detected in 65.6%, 43.7%, and 40.6% of the cases, respectively. The red complex was detected in 15.6% of samples taken from acute periradicular abscesses.

“Red complex”is directly associated with the severity of the periodontal disease, but limited information is available on the role of red complex in the endodontic lesion. Therefore, we have taken the red complex as an experimental group.[15]

Isabela N et al.[16] assessed the occurrence of the red complex in root canal infections through the use of a sensitive technique- the 16S rDNA–directed PCR. Samples were obtained from 50 necrotic pulps with periradicular pathosis. Ten cases were diagnosed as acute periradicular abscesses. DNA was extracted from the samples and analyzed with a PCR-based identification assay. It was concluded that at least 1 member of the red complex was found in 33 of 50 cases. T denticola, P gingivalis, and B forsythus were detected in 44%, 30%, and 26% of the cases, respectively. The red complex was found in 4 of 50 cases. No particular signs or symptoms were associated with the presence of these bacterial species. However, because the bacterial species from the red complex are recognized oral pathogens, their occurrence in root canal infections suggests that they may play a role in the pathogenesis of periradicular diseases.

In the present study, E. faecalis was found to be significantly associated with pain in primary and secondary infections. The present study was not in accordance with the study conducted by R. V. Vineet et al.[4] that postulated the significant association of S. mitis with pain in primary root canal infections.

Kemthong Mitrakul conducted a study to evaluate the presence of Lactobacilli and  Enterococcus faecalis Scientific Name Search s in root canal infections of primary teeth and analyzed the association between clinical signs and symptoms with the presence of these bacterias. It was concluded that the bacterial levels of total bacteria, Lactobacillus and E. faecalis in the pulp necrosis group were higher than the irreversible pulpitis group. Levels of Lactobacillus and E. faecalis were associated with a history of pain, pathologic finding of lamina dura, and furcation involvement. Gingival swelling was correlated with Lactobacillus quantities.[17]

Paulo Nelson-Filho et al. compared the Gram-negative pathogens identified in the root canals of primary teeth with irreversible inflammatory pulpitis and in teeth showing apical periodontitis. It was concluded that a higher number of Gram-negative bacteria were found in teeth with apical periodontitis compared to teeth with irreversible inflammatory pulpitis.[18]

Isabela N. Rocas et al. Samples were taken from the root canals of teeth with asymptomatic apical periodontitis (n = 73) and by aspiration of purulent exudate from acute abscesses (n = 55). Bacterial DNA was detected in all cases. In abscesses, the most prevalent taxa were Fusobacterium nucleatum Scientific Name Search  (60%),  Porphyromonas endodontalis Scientific Name Search 3%), Parvimonas Micra (51%), and Streptococcus species (45%). The most frequently detected taxa in asymptomatic teeth were P. endodontalis (63%),  Dialister invisus Scientific Name Search %), Olsenella uli (56%), and F. nucleatum (51%). However, semiquantitative data demonstrated that P. endodontalis, Prevotella baroniae, Treponema denticola, and Streptococcus species were significantly more frequent at levels >105 in abscesses than in asymptomatic cases (P < 0.05).[19]

Pradeep Shetty et al. evaluated and compared the anaerobic microflora (obligate and facultative) of the root canals of nonvital teeth with periapical lesions, in diabetic (Type II) and nondiabetic patients. Obligate anaerobes were significantly more associated with cases of Type II diabetes mellitus, suggesting the role of these microorganisms in modulating the inflammatory and immunologic responses as well as its level in root canal infections, which can influence the pain threshold of the patient.[20]

Foschi et al.[21] performed a study to analyze the role of T. denticola as a monoinfection as well as a part of “red complex” infection in the causation of endodontic infections into 8 weeks old severe combined immunodeficiency mice. Study results demonstrated periapical bone resorption in T. denticola monoinfection when compared to the red complex.

T. denticola was detected in 15/60 cases with periapical lesions of which five were tender on percussion. The red complex was detected in 10% (6/60) samples taken from acute periradicular abscess samples. These observations are suggestive of the role of “red complex” in the pathogenesis of acute periradicular abscesses. These findings have been corroborated by Sanghavi et al.[22] using the multiplex PCR technique.

In continuously changing environmental conditions bacteria form multiple complexes to survive, and the relationships between bacterial species and hosts can provide a better understanding of disease processes. Synergistic bacterial interactions allow different species to coexist in habitats in which interacting bacteria do not exist alone and increase the possibility of survival. Bacterial synergism may be another significant factor in the development of symptoms and signs of endodontic origin and the presence of some pathogenic species in the root canal could provide an environment for the formation of acute periradicular inflammation.[23],[24]

Implications for primary care

Endodontic disease refers to an infection of the root canal space and is a significant cause of dental morbidity worldwide. Endodontic treatment or root canal treatment serves to eliminate the microbial biofilm and prevents reinfection of the root canal space. The primary objective of endodontic therapy is to create a biologically acceptable environment within the root canal system that allows for the healing and maintenance of the health of the periradicular tissue. Bacteria are one of the main causes of pulp problems, and they have different methods of penetrating and invading the endodontic space such as through carious lesions, traumatic pulp exposures, and fractures. The types of bacteria found to range from facultative anaerobes to aerobes, up to the most resistant species able to survive in nutrient-free environments; the bacterial species Enterococcus faecalis belongs to this last group. Enterococcus faecalis is considered one of the main causes of recurring apical periodontal lesions following endodontic treatment, with persistent lesions occurring even after retreatment.[25],[26]


  Conclusion Top


The high prevalence of red complex bacteria in the present study suggests that these bacteria can be correlated with the etiopathogenesis of periradicular diseases. Identification of such microorganisms help in treating the endodontic infections at an early stage.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Ashraf FF, Barry F, Caimano M, Clawson M, Zhu Q, Carver R, et al. PCR-based identification of bacteria associated with endodontic infections. J Clin Microbiol 2002;5:3223-323.  Back to cited text no. 1
    
2.
Haapasalo M. Black-pigmented Gram-negative anaerobes in endodontic infections. FEMS Immunol Med Microbiol 1993;6:213-8.  Back to cited text no. 2
    
3.
Pecuikine V, Maneliene R, Balcikouyte E, Drukteinis S, Rutkunas V. Microorganisms in root canal infections: A review. Stomatologiya, Baltic Dental Maxillofas J 2008;10:4-9.  Back to cited text no. 3
    
4.
Vineet RV, Nayak M, Kotigadde S. Association of endodontic signs and symptoms with root canal pathogens: A clinical comparative study. Saudi Endod J 2016;6:82-6.  Back to cited text no. 4
  [Full text]  
5.
Schirrmeister JF, Liebenow AL, Braun G, Wittmer A, Hellwig E, Al-Ahmad A. Detection and eradication of microorganisms in root-filled teeth associated with periradicular lesions: An in vivo study. J Endod 2007;33:536-40.  Back to cited text no. 5
    
6.
Gomes BP, Lilley JD, Drucker DB. Associations of endodontic symptoms and signs with particular combinations of specific bacteria. Int Endod J 1996;29:69-75.  Back to cited text no. 6
    
7.
Hess JC, Victor M. Thérapeutiques écologiques des canaux. Chir Dent Fran 1983;186:48-52.  Back to cited text no. 7
    
8.
Drucker DB. Microbial ecology of the dental root canal. Microb Ecol Health Dis 2000;12:160-9.  Back to cited text no. 8
    
9.
Pirani C, Bertacci A, Cavrini F, Foschi F, Acquaviva GL, Prati C, et al. Recovery of Enterococcus faecalis in root canal lumen of patients with primary and secondary endodontic lesions. New Microbiol 2008;31:235-40.  Back to cited text no. 9
    
10.
Martina LP, Ebenezar AR, Ghani MF, Narayanan A, Sundaram M, Mohan AG. An in vitro comparative antibacterial study of different concentrations of green tea extracts and 2% chlorhexidine on Enterococcus faecalis. Saudi Endod J 2013;3:120-4.  Back to cited text no. 10
    
11.
Mindere A, Kundzina R, Nikolajeva V, Eze D, Petrina Z. Microflora of root-filled teeth with apical periodontitis in Latvian patients. Stomatologija 2010;12:116-21.  Back to cited text no. 11
    
12.
Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: Its role in root canal treatment failure and currentconcepts in retreatment. J Endod 2006;32:93-8.  Back to cited text no. 12
    
13.
Rôças IN, Siqueira JF Jr, Santos KR. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod 2004;30:315-20.  Back to cited text no. 13
    
14.
Ozbek SM, Ozbek A. Real-time polymerase chain reaction of ''red complex'' (Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) in periradicular abscesses. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;110:670-4.  Back to cited text no. 14
    
15.
Sanghavi, TH, Shah N, Shah RR, Sanghavi A. Investigate the correlation individually or as a ''Red complex'' by a multiplex PCR method. J Conserv Dent 2014;17:555-60.  Back to cited text no. 15
    
16.
Rôças, IN, Siqueira JF Jr, Santos KRN, Coelho AMA, Rio de Janeiro. ''Red complex'' (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola) in endodontic infections: A molecular approach. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001;91:468-71.  Back to cited text no. 16
    
17.
Mitrakul K, Vongsawan K, Watcharakirin W, Khererat P. Quantitative analysis of lactobacillus and enterococcus faecalis between irreversible pulpitis and pulp necrosis in primary teeth. Dental Res Oral Health 2019;2:017-031.  Back to cited text no. 17
    
18.
Nelson-Filho P, Ruviere DB, Mussolino de Queiroz A, Garcia de Paula-Silva FW, Bezerra da Silva RA, et al. Comparative Molecular Analysis of Gram-Negative Bacteria in Primary Teeth with Irreversible Pulpitis or Periapical Pathology. Pediatr Dent 2018;40:259-64.  Back to cited text no. 18
    
19.
Rocas IN, Siqueira JF Jr. Frequency and levels of candidate endodontic pathogens in acute apical abscesses as compared to asymptomatic apical periodontitis. PLoS One 2018;13:e0190469.  Back to cited text no. 19
    
20.
Shetty P, Chawla M, Mulay S, Bhargava K, Kumar T, Vidhya V. A comparative study of isolation of obligate anaerobes and facultative anaerobes in microbial flora of non- vital teeth in diabetic (Type II) and non-diabetic patients. Int J Curr Res Rev 2018;10:13-7.  Back to cited text no. 20
    
21.
Foschi F, Izard J, Sasaki H, Sambri V, Prati C, Muller R, et al. Treponema denticola in disseminating endodontic infections. J Dent Res 2006;85:761-5.  Back to cited text no. 21
    
22.
Sanghavi TH, Shah N, Shah RR, Sanghavi A. Investigate the correlation between clinical signs and symptoms and the presence of P. gingivalis, T. denticola, and T. forsythia individually or as a ''Red complex'' by a multiplex PCR method. J Conserv Dent 2014;17:555-60.  Back to cited text no. 22
    
23.
Rocas IN, Siqueira JF Jr. Root canal microbiota of teeth with chronic apical periodontitis. J Clin Microbiol 2008;46:3599-606.  Back to cited text no. 23
    
24.
Rocas IN, Siqueira JF Jr, Santos KR, Coelho AM. ''Red complex'' (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola) in endodontic infections: A molecular approach. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001;91:468-71.  Back to cited text no. 24
    
25.
Dioguardi M, Di Gioia G, Illuzzi G, Arena C, Alberto Caponio VC, Caloro GA, et al. Inspection of the Microbiota in Endodontic Lesions. Dent J 2019;7:1-15.  Back to cited text no. 25
    
26.
Abusrewil S, Alshanta OA, Albashaireh K, Alqahtani S, Nile CJ, Scott JA, et al. Detection, treatment, and prevention of endodontic biofilm infections: What's new in 2020? Crit Rev Microbiol 2020;46:194-212.  Back to cited text no. 26
    



 
 
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