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ORIGINAL ARTICLE
Year : 2020  |  Volume : 9  |  Issue : 8  |  Page : 4368-4374

Comparison of expression of myofibroblasts in normal oral mucosa, oral epithelial dysplasia, and oral squamous cell carcinoma using α-SMA and vimentin: An immunohistochemical study


1 Department of Oral Pathology and Microbiology, Swami Devi Dyal Hospital and Dental College, Golpura, Barwala, Panchkula, Haryana, India
2 Department of Medical, Health and Family Welfare, Uttarakhand, India
3 Department of Public Health Dentistry, Dental Institute, RIMS, Ranchi, Jharkhand, India
4 Department of Conservative, Endodontics and Aesthetic Dentistry, Dental Institute, RIMS, Ranchi, Jharkhand, India
5 Department of Oral Pathology and Microbiology, Dasmesh Institute of Research and Dental Sciences, Faridkot, Punjab, India

Correspondence Address:
Dr. Harkanwal P Singh
Associate Professor, Department of Oral Pathology and Microbiology, Dasmesh Institute of Research and Dental Sciences, Faridkot-151203, Punjab
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jfmpc.jfmpc_172_20

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Background: Myofibroblasts are thought to play critical roles in inflammation, growth, repair, premalignancy, and malignancy. This study was done to evaluate, compare and co- relate the progressive increase in the immunohistochemical expression of myofibroblasts in normal oral mucosa, epithelial dysplasia, and oral squamous cell carcinoma (OSCC). To compare and co-relate the expression of myofibroblasts in normal oral mucosa, epithelial dysplasia, and oral squamous cell carcinoma. To co-relate the progressive increase in myofibroblasts expression in normal oral mucosa, epithelial dysplasia, and oral squamous cell carcinoma. Materials and Method: Forty-nine paraffin-embedded tissue blocks with 7 cases of normal oral mucosa, 21 cases of epithelial dysplasia, and 21 diagnosed cases of OSCCs were studied. The samples were subjected to heat-induced antigen retrieval methods followed by staining using primary mouse monoclonal antibodies against α-smooth muscle actin (SMA) and vimentin. Staining index of all the sections was calculated. Statistical analysis was performed using the Kruskal–Wallis test, Mann–Whitney U test, and Chi-square test. Values of P less than or equal to 0.05 (P ≤ 0.05) were considered statistically significant. Results: Statistically significant staining index was obtained by α-SMA and vimentin between normal oral mucosa, epithelial dysplasia, and OSCC. Conclusion: Myofibroblast may play a role only during initial tumorigenesis that is the conversion of severe dysplasia into OSCC.


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