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Evaluation and diagnostic usefulness of saliva for detection of HIV antibodies: A cross-sectional study
Puneeta Vohra1, Vikram Belkhode2, Sharayu Nimonkar2, Suraj Potdar3, Rishabh Bhanot4, Izna5, Rahul Vinay Chandra Tiwari6
1 Department of Oral Medicine and Radiology, Faculty of Dental Sciences, S.G.T. University, Gurugram, Haryana, India
2 Department of Prosthodontics, Sharad Pawar Dental College and Hospital, Sawangi, Wardha, Maharashtra, India
3 Department of Orthodontics and Dentofacial Orthopedics, Vasantdada Patil Dental College and Hospital, Sangli - Tasgaon Rd, Kavalapur, Maharashtra, India
4 Consultant Oral and Maxillofacial Surgeon, Jyoti Kendra General Hospital, Ludhiana, Punjab, India
5 Department of Microbiology, Government Medical College and Associated Hospital Rajouri, Jammu and Kashmir, India
6 Consultant Oral and Maxillofacial Surgeon, CLOVE Dental and OMNI Hospitals, Visakhapatnam, Andhra Pradesh, India
Correspondence Address:
Dr. Izna
Department of Microbiology, Government Medical College and Associated Hospital Rajouri, Jammu and Kashmir
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jfmpc.jfmpc_138_20
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Background: Use of saliva as a specimen for detection of antibodies to infectious agents has generated particular interest in AIDS research community since 1980s. HIV specific antibodies of immunoglobulin isotypes IgA, IgG, and IgM are readily found in salivary secretions. Aim and Objectives: In the present study, HIV specific antibodies were detected in saliva and serum samples of HIV patients by ELISA in confirmed HIV seropositive patients and efficacy of saliva was established in diagnosis of HIV. Methods: The 100 saliva and serum samples were collected from age and sex matched confirmed HIV seropositive subjects and 100 Healthy Controls without any infections. HIV antibodies were determined by enzyme-linked immunosorbent assay (ELISA) using Genscreen HIV 1/2 Kit. Results: The results were found to be 99% sensitive and 100% specific for saliva samples, while it was 100% sensitive and specific for serum samples. Conclusion: Saliva can be used as alternative to blood for detection of HIV antibodies as saliva collection is painless, non-invasive, inexpensive, simple, and rapid. Salivary antibody testing may provide better access to epidemic outbreaks, children, large populations, hard-to-reach risk groups and may thus play a major role in the surveillance and control of highly infectious diseases.
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